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PT (Prothrombin Time)
Prothrombin Time manual procedure in minimum word. (Click here)
PT (Prothrombin Time)
1) Pre warm the PT reagent 10 minutes at 37°C.
2) Take 100 ul of plasma into the test tube wait for 30 seconds.
3) Add 200 ul of reagent in the test tube. Put it the water bath and start the stop watch. Stop it when the clot is formed take out the tube and look for the visible clot.
*Calculate the INR on the system.
The Prothrombin Time (PT) test, also known as the protime test, is a laboratory blood test that measures the time it takes for blood to clot. It evaluates the activity of certain coagulation factors in the blood, particularly prothrombin, which is a key factor in the clotting process.
The PT test is commonly used to monitor the effectiveness of anticoagulant therapy, such as warfarin, by measuring the clotting time in relation to a reference range. It helps healthcare providers determine the appropriate dosage of medication to prevent excessive blood clotting or bleeding.
The test involves taking a blood sample from a vein, usually in the arm, which is then mixed with a substance called a reagent that initiates the clotting process. The time it takes for the blood to clot is measured and compared to a control or reference value. The result is reported as the international normalized ratio (INR) or as the ratio of the patient's clotting time to that of a control sample.
Abnormal PT/INR values can indicate various conditions, including liver disease, vitamin K deficiency, certain genetic disorders affecting clotting factors, or the presence of anticoagulant medications. Results outside the normal range may require further evaluation and adjustment of treatment.
It's important to note that the interpretation of PT/INR results should be done by a qualified healthcare professional who will consider the patient's medical history, medication regimen, and other relevant factors to make informed decisions regarding treatment and management.
APTT (Activated Partial Thromboplastin Time)
APTT (Activated Partial Thromboplastin Time) manual procedure in minimum word. (Click here)
APTT (Activated Partial Thromboplastin Time)
1) Separate test tube contain in Cephascreen and Calcium Chloride solution should be brought to 37°C (keep in water bath 5 - 10 minutes)
2) To test tube add 100 ul plasma and 100 ul of Cephascreen shake tube briefly. To mix the reagent and plasma and place tube at 37°C for 3 - 5 minutes.
3) Add Forcibly 100 ul pre warmed Calcium chloride into plasma and simultaneously start the stopwatch. Shake tube keep at 37°C for 20 seconds. Incubation remove the tube. Gently tilt back and forth until a gel clot form stop the watch and record time.
The activated partial thromboplastin time (APTT) test is a laboratory blood test used to assess the overall functioning of the intrinsic pathway of the coagulation cascade. It measures the time it takes for blood to clot in response to certain activators.
The APTT test is primarily used to monitor the effectiveness of heparin therapy, a medication that prevents blood clots from forming or growing. It helps healthcare providers determine the appropriate dosage of heparin to achieve the desired anticoagulant effect.
During the APTT test, a blood sample is collected from a vein and mixed with an activator that triggers the clotting process. The time it takes for the blood to clot is measured and compared to a reference range. The result is reported as the number of seconds it took for the clot to form.
The APTT test measures the activity of several coagulation factors involved in the intrinsic pathway, such as factors VIII, IX, XI, and XII. It is used to screen for and monitor various bleeding disorders, such as hemophilia and von Willebrand disease.
Abnormal APTT results can indicate an increased risk of bleeding or clotting. If the APTT is prolonged, it may suggest a deficiency or dysfunction of one or more clotting factors. In contrast, a shortened APTT may be associated with an increased risk of blood clot formation.
Interpretation of APTT results should be done by a qualified healthcare professional who will consider the patient's medical history, medication regimen, and other relevant factors to make informed decisions regarding treatment and management.
It's important to note that the APTT test is just one component of a comprehensive evaluation of a patient's coagulation profile, and it is typically interpreted in conjunction with other tests, such as the prothrombin time (PT) and platelet count.
